|Year : 2018 | Volume
| Issue : 1 | Page : 29-33
Does pretrigger echogenic endometrium in assisted reproductive technology cycles reflect raised serum progesterone level?
Monna Pandurangi1, N Sanjeeva Reddy1, G Usha Rani2, Hepzibha Kirubamani3
1 Department of Reproductive Medicine and Surgery, Sri Ramachandra Medical College, Chennai, Tamil Nadu, India
2 Department of Obstetrics and Gynaecology, Sri Ramachandra Medical College, Chennai, Tamil Nadu, India
3 Department of Obstetrics and Gynaecology, Saveetha Medical College, Chennai, Tamil Nadu, India
|Date of Web Publication||27-Mar-2018|
Dr. Monna Pandurangi
Department of Reproductive Medicine and Surgery, Sri Ramachandra Medical College and Research Institute, Sri Ramachandra University, Ramachandra Nagar, Porur, Chennai - 600 116, Tamil Nadu
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Background: Serum progesterone is the main hormone of the luteal phase. The hyperechoic pattern of the endometrium in the luteal phase is believed to be induced by raised serum progesterone. Serum progesterone is found to be raised cases of controlled ovarian stimulation (COS) cycle on the day of ovulation trigger. Aim: This study aims to find the association between echogenicity of endometrium and raised serum progesterone Objective: The objective of this study is to determine whether raised pretrigger serum progesterone influences the echogenicity of the endometrium Materials and Methods: In this prospective observational study, we evaluated 221 patients who underwent COS. Echogenic patterns of the endometrium on transvaginal sonography were described as hypoechoic/trilaminar endometrium (Type A), isoechoic (Type B), and hyperechoic (Type C). The endometrial pattern and serum progesterone levels were evaluated on the day of ovulation trigger and value of ≥1 ng/ml was considered as elevated. Results: A total of 168 patients out of 221 patients (76.01%) had elevated serum progesterone levels on the day of ovulation trigger. Type A endometrium was found in a total of 174 patients, of these 132 patients (75.86%) had raised serum progesterone. Type B endometrium was found in 35 patients, of these 27 patients (77.14%) had raised serum progesterone. Type C endometrium was seen in 12 patients, out of these 9 patients (75.00%) had raised serum progesterone level. There was no statistically significant difference in the echogenic patterns of endometrium in patients with raised progesterone (≥1 ng/ml). On intergroup comparison, the difference in the progesterone levels between type A and type C was statistically significant (P = 0.02), and on receiver operating characteristic curve analysis, echogenic endometrium was found to predict progesterone level of 1.57 ng/ml with a sensitivity of 58.3% and specificity of 58.4% only. Conclusion: Echogenicity of the endometrium does not reliably predict raised serum progesterone on the day of ovulation trigger.
Keywords: Assisted reproductive technology, echogenicity, endometrium, in vitro fertilization, progesterone, transvaginal sonography
|How to cite this article:|
Pandurangi M, Reddy N S, Rani G U, Kirubamani H. Does pretrigger echogenic endometrium in assisted reproductive technology cycles reflect raised serum progesterone level?. J Hum Reprod Sci 2018;11:29-33
|How to cite this URL:|
Pandurangi M, Reddy N S, Rani G U, Kirubamani H. Does pretrigger echogenic endometrium in assisted reproductive technology cycles reflect raised serum progesterone level?. J Hum Reprod Sci [serial online] 2018 [cited 2023 Mar 24];11:29-33. Available from: https://www.jhrsonline.org/text.asp?2018/11/1/29/228601
| Introduction|| |
Identification of optimal conditions in controlled ovarian stimulation (COS) and embryo transfer (ET) is of substantial clinical interest to increase the pregnancy rates in assisted reproductive technology (ART) cycles. Advancements in fertilization methods and embryo culture techniques have led to only marginal improvement in the pregnancy rates. Endometrial receptivity has been the focus of interest recently, and its disorders are likely to represent an important cause of the suboptimal embryo implantation rates.
In an ART cycle, direct assessment of the endometrial receptivity by endometrial biopsy is impossible in the ET cycles. As an alternative to endometrial biopsies, high-resolution transvaginal sonography (TVS) makes it possible to monitor noninvasively the endometrium through TVS assessment of the pattern of the endometrium wherein the endometrial echogenicity is assessed., Patterns of endometrium were graded as Type A-hypoechoic/trilaminar (hypoechoic endometrium with prominent central and outer echogenic lines), Type B-isoechoic (same reflectivity of the endometrium as the surrounding myometrium and a poorly defined central echogenic line), and Type C-hyperechoic (increased reflectivity compared to myometrium and the central and the outer echogenic line not differentiated clearly. It is well established that in the proliferative phase, the endometrium has a triple line/hypoechoic endometrium (Type A) and this appearance changes in the secretory phase, becoming hyperechoic (Type C). Serum Progesterone is the main hormone of the luteal phase and the hyperechoic pattern of the endometrium in the luteal phase is believed to be due to changes in the endometrium induced by raised serum progesterone. Recently, serum progesterone is found to be elevated during the late follicular phase in as many as 20—38% of ART cycles and is reported to decrease the pregnancy rates by interfering with implantation. We were interested to determine whether the hyperechoic (Type C) endometrium as detected by TVS on the day of ovulation trigger in some patients, be a reliable ultrasound marker of raised serum progesterone during the late follicular phase. The effect of raised serum progesterone on the endometrial echogenicity in the late follicular phase is controversial. There are few studies stating that follicular phase echogenicity of the endometrium is due to raised serum progesterone but few others refute this proposition., We analyzed 221 ART cycles to determine if raised serum progesterone (≥1 ng/ml) in the late follicular phase was associated with increased echogenicity of the endometrium.
| Materials and Methods|| |
We conducted a prospective cohort study of patients who underwent COS for in vitro fertilization in the Department of Reproductive Medicine and Surgery at a tertiary care university teaching hospital, from January 2013 to December 2016. Institutional Ethical Committee clearance was obtained (Ref-IEC/12/MAR/94/09). A total of 221 patients were included in the study after obtaining written and informed consent. Patients in whom serum progesterone levels were not estimated, those with a history of pelvic tuberculosis, Asherman's syndrome, adenomyosis, and with retroverted fixed uterus, were excluded from the study. Patients underwent COS with flexible GnRH antagonist protocol. COS was started from day 2 or day 3 of menstrual cycle (spontaneous or withdrawal to combined estrogen-progesterone pill). Recombinant follicle stimulating hormone (rFSH) with or without human menopausal gonadotropin was started, dose individualized as per departmental protocol. When at least two follicles were 18 mm in diameter ovulation trigger was given with recombinant human chorionic gonadotropin 250 mcg. On the day of ovulation, trigger echogenicity of the endometrium was assessed by noting the endometrial pattern by TVS using a transvaginal probe 9 MHZ Ultrasoniox-SonixOP ultrasound system and the pattern were graded as hypoechoic/trilaminar (hypoechoic endometrium with central and outer echogenic lines-Type A), isoechoic (same reflectivity of the endometrium as the surrounding myometrium and poorly defined central echogenic line-Type B), and hyperechoic (increased reflectivity compared to myometrium and the central and the outer echogenic line not differentiated clearly-Type C). Serum Estradiol and Progesterone levels were estimated (using ADVIA Centaur XP system by chemiluminescence method with analytical sensitivity for serum estradiol of 7.0 pg/ml and for serum progesterone 0.21 ng/ml) before administration of ovulation trigger. Serum progesterone levels of ≥1 ng/ml on the day of ovulation trigger was considered to be elevated in our study. Oocyte retrieval was performed approximately 35–36 h after ovulation trigger. ICSI was performed in all the cases. Fresh ET was done on the 3rd or the 5th day after oocyte retrieval or underwent freeze-all and subsequent frozen ET, as per department protocol. Serum β-human chorionic gonadotropin (hCG) concentrations were estimated on the 14th day of ET and a value of >5 mIU was considered positive for pregnancy.
The collected data were analyzed with Statistical Package for Social Sciences for Windows, Version 16.0 (SPSS Inc., Chicago, IL, USA). To describe about the data descriptive statistics frequency analysis, percentage analysis was used for categorical variables and the mean and standard deviation were used for continuous variables. For the multivariate analysis, the one way ANOVA was used. Pearson's correlation was used to assess the relationship between the variables. Chi-square test was used to find the significance in categorical data. In all of the above statistical tools, the P < 0.05 was considered as statistically significant.
| Results|| |
A total of 221 women undergoing COS for ART were evaluated. Indications for COS are tabulated in [Table 1]a. Male factor which accounted for 43.43% followed by tubal factor which accounted for 24.85% were the most common indications. Demographic and COS details are summarized in [Table 1]b. There was no statistically significant difference in the distribution of patient, as per age and body mass index between the three groups. The COH details such as duration of stimulation, total dose of gonadotropins, and total dose of rFSH and HMG was not statistically different between Type A, Type B, and Type C endometrium. Pretrigger serum estradiol, endometrial thickness, and the number of oocytes obtained in all the three groups also did not show any statistical difference between all the three groups.
Out of 221, 127 patients had fresh ET, 50 patients conceived (39.3%). Pregnancy rates in patients with Type A, Type B, and Type C endometrium on the day of hCG trigger were 42.1%, 30%, and 25%, respectively. There was no statistical difference between the pregnancy rates between all the three groups.
The pattern of endometrium was not found to be significantly associated with elevated pretrigger serum progesterone as shown in [Table 2]a-c. However, in the intergroup analysis, the pretrigger serum progesterone levels were significantly higher in patients with compared to Type A – 2.41 ± 2.7 ng/ml versus 1.60 ± 0.8 ng/ml, P = 0.02 [Table 2]c. Receiver operating characteristic curve was plotted for the values of progesterone in patients with Type C endometrium, to know the reliability of endometrium as a predictor of elevated progesterone level. On analysis, the echogenic endometrium (type C) is found to reliably predict elevated progesterone level at 1.57 ng/ml, with a sensitivity of 58.3% and specificity of 58.4% [Table 2]d.
| Discussion|| |
The impact of premature serum progesterone elevation at the end of the follicular phase in COS cycle for in vitro fertilization is extensively studied. Most of the studies have reported lower pregnancy rates in patients with high progesterone concentration on the day of ovulation trigger. Premature progesterone elevation does not alter the oocyte quality has been proved by many studies  while there are accumulated data suggesting its negative impact on the endometrium. Elevated progesterone levels induce premature endometrial maturation (secretory changes), and as a consequence, there is an earlier opening of the implantation window that leads to lack of synchronization of the crosstalk between embryo and endometrium. The elevated serum progesterone on the day of ovulation trigger is known to cause premature maturation changes in the endometrium. However, does this raised serum progesterone in the follicular phase result in echogenic endometrium as seen during the luteal phase?
In our study, we have found that preovulatory progesterone raise did not significantly increase the endometrial echogenicity, as is known to occur with luteal phase progesterone. A similar study was conducted by Corbacioglu et al. where the authors did not find any significant association between various patterns of endometrium and serum progesterone concentrations. The mean concentration of serum progesterone in their study was 1.5 ± 1.38 ng/ml in trilaminar endometrium, 1.81 ± 1.8 ng/ml in isoechogenic endometrium and 1.12 ± 0.59 ng/ml in hyperechogenic endometrium (P = 0.25). In the present study, the mean serum levels were 1.60 ± 0.85 in Type A, 1.78 ± 1.15 ng/ml in Type B, and 2.41 ± 2.37 ng/ml in Type C. In our study, although the difference in the mean progesterone value between Type A and Type C endometrium was significant, the echogenic endometrium could not reliably predict raised progesterone between 1 and 1.56 ng/ml range. Only at serum progesterone level of 1.57 ng/ml, the type C endometrium has the highest predictability of elevated progesterone, but the sensitivity and specificity are very low – 58.3% and 58.4%, respectively. Thus, in our study, type C endometrium could not reliably predict elevated pretrigger serum progesterone. In a study by Julian A. Gingold et al., hyperechoic endometrium on the of trigger day, showed significantly high progesterone value (1.21 ± 0.5 ng/ml) than the other patterns with a lower implantation rate but again with a limited statistical power <50%.
Fanchin et al. studied the endometrial echogenicity values on the day of hCG administration, oocyte retrieval and ET in two groups of patient those with serum progesterone ≤0.9 ng/ml and with serum progesterone values >0.9 ng/ml. They found that on the day of hCG administration, the degree of endometrial echogenicity was similar in both groups (41% vs. 40%), but after hCG administration, it increased significantly faster in the high progesterone group than in the low progesterone group (70% vs. 63% at oocyte retrieval and 90% vs. 79% at ET, respectively).
On studying the transformation induced by progesterone during the normal spontaneous menstrual cycle, stromal edema is observed relatively late (days 21–22), whereas endometrial echogenicity often starts to increase earlier, during the first days of exposure to progesterone. Therefore, it is conceivable that other histologic events, such as glandular coiling (day 16 onward) and/or secretion (day 17 onward), contribute to increase the endometrial echogenicity. The absence of hyperechogenicity due to raised progesterone in the follicular phase may be explained by the fact that the magnitude of the histologic transformation triggered by progesterone on the endometrium may be still too incipient at this phase of COS to be discerned clearly by ultrasonography.
Ubaldi et al. performed endometrial biopsy on the day of oocyte retrieval and compared the difference in the secretory changes between patients with normal progesterone (P) ≤0.9 ng/ml Group 1 and in those with raised progesterone (P) of ≥1.1 ng/ml (Group 2). In group II, the endometrial maturation was advanced by 0.7 days (P < 0.01) more than the normal P group. Although advancement of endometrial maturation was significantly different between groups I and II, it was less than that might be expected from the number of days of premature progesterone rise and cumulative exposure to this. They speculated that it might be due to insufficient progesterone receptor priming of the endometrium at the time of premature progesterone elevation.
The lack of correlation between circulating progesterone levels and follicular phase echogenicity, observed in previous investigations and confirmed by the present study raises the question of other factors which would alter the echogenicity of the endometrium during the follicular phase. Corbacioglu et al. found that older patients and patients in whom the total gonadotropin dose was higher were more in the hyperechoic group. They also suggested that the other factors such as serum androgens may be responsible for the echogenicity. In our study, we tried to correlate the effect of age and total dose of gonadotropin levels on the echogenicity of the endometrium, but no significant association with any of the above factors were noted [Table 1]b. However, one of the major limitations of our study is the small number of patients in the echogenic endometrium (Type C) group.
| Conclusion|| |
Elevated pretrigger progesterone does not result in a significant change in the endometrial echogenicity. Further large prospective controlled studies are required to identify the factors responsible for endometrial echogenicity in the follicular phase.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| References|| |
Strowitzki T, Germeyer A, Popovici R, von Wolff M. The human endometrium as a fertility-determining factor. Hum Reprod Update 2006;12:617-30.
Barker MA, Boehnlein LM, Kovacs P, Lindheim SR. Follicular and luteal phase endometrial thickness and echogenic pattern and pregnancy outcome in oocyte donation cycles. J Assist Reprod Genet 2009;26:243-9.
Dede H, Dilbaz S, Demir B, Çınar O, Dede S, Goktolga U. The effect of endometrial thickness and pattern on pregnancy outcome in IVF/ICSI cycles. Platin Glob J Med Med Sci 2015;X:1-6.
Bosch E, Labarta E, Crespo J, Simón C, Remohí J, Jenkins J, et al
. Circulating progesterone levels and ongoing pregnancy rates in controlled ovarian stimulation cycles for in vitro
fertilization: Analysis of over 4000 cycles. Hum Reprod 2010;25:2092-100.
Zhao J, Zhang Q, Li Y. The effect of endometrial thickness and pattern measured by ultrasonography on pregnancy outcomes during IVF-ET cycles. Reprod Biol Endocrinol 2012;10:100.
Li R, Qiao J, Wang L, Zhen X, Lu Y. Serum progesterone concentration on day of HCG administration and IVF outcome. Reprod Biomed Online 2008;16:627-31.
Gingold JA, Lee JA, Rodriguez-Purata J, Whitehouse MC, Sandler B, Grunfeld L, et al.
Endometrial pattern, but not endometrial thickness, affects implantation rates in euploid embryo transfers. Fertil Steril 2015;104:620-8.e5.
Corbacioglu A, Baysal B. Effects of steroid hormone levels on the ultrasound appearance of the preovulatory endometrium in controlled ovarian hyperstimulation cycles. Int J Fertil Steril 2012;5:203-6.
Legro RS, Ary BA, Paulson RJ, Stanczyk FZ, Sauer MV. Premature luteinization as detected by elevated serum progesterone is associated with a higher pregnancy rate in donor oocyte in-vitro
fertilization. Hum Reprod 1993;8:1506-11.
Labarta E, Martínez-Conejero JA, Alamá P, Horcajadas JA, Pellicer A, Simón C, et al.
Endometrial receptivity is affected in women with high circulating progesterone levels at the end of the follicular phase: A functional genomics analysis. Hum Reprod 2011;26:1813-25.
Haouzi D, Bissonnette L, Gala A, Assou S, Entezami F, Perrochia H, et al.
Endometrial receptivity profile in patients with premature progesterone elevation on the day of HCG administration. Biomed Res Int 2014;2014:951937.
Fanchin R, Righini C, Olivennes F, Taieb J, de Ziegler D, Frydman R, et al.
Computerized assessment of endometrial echogenicity: Clues to the endometrial effects of premature progesterone elevation. Fertil Steril 1999;71:174-81.
Ubaldi F, Bourgain C, Tournaye H, Smitz J, Van Steirteghem A, Devroey P, et al.
Endometrial evaluation by aspiration biopsy on the day of oocyte retrieval in the embryo transfer cycles in patients with serum progesterone rise during the follicular phase. Fertil Steril 1997;67:521-6.
[Table 1], [Table 2]
|This article has been cited by|
||The implications of the purinergic signaling throughout pregnancy
| ||Lucas Sagrillo-Fagundes, Thaís Casagrande Paim, Luiza Pretto, Isadora Bertaco, Carla Zanatelli, Cathy Vaillancourt, Márcia R. Wink |
| ||Journal of Cellular Physiology. 2021; |
|[Pubmed] | [DOI]|
||Premature Luteinization May Impact In Vitro Fertilization Outcomes Through Dual Action on Both Uterus and Embryos
| ||Reda S. Hussein, Elizabeth A Stewart, Yulian Zhao |
| ||Current Obstetrics and Gynecology Reports. 2021; 10(2): 17 |
|[Pubmed] | [DOI]|