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| ORIGINAL ARTICLE |
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| Year : 2022 |
Volume
: 15 | Issue : 1 | Page
: 21-26 |
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An In vitro study on the protective effect of melatonin on human sperm parameters treated by cadmium
Sekineh Amirjannaty1, Nasrin Ghanami Gashti2, Ali Mojtahedi3, Asieh Ashouri4, Mohammad Hadi Bahadori5
1 Student Research Committee, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
2 Reproductive Health Research Center, Department of Obstetrics & Gynecology, Al-Zahra Hospital, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
3 Microbiology Department, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
4 Department of Health, Education and Promotion School of Health, Health and Environment Research Center, Guilan University of Medical Sciences, Rasht, Iran
5 Cellular and Molecular Research Center, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran
Correspondence Address:
Dr. Mohammad Hadi Bahadori
Cellular and Molecular Research Center, School of Medicine, Guilan University of Medical Sciences, Rasht
Iran
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/jhrs.jhrs_151_21
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Background: Male infertility account for nearly 50% of infertility cases. Cadmium is regarded as a well-known toxic metal for industrial applications; high amounts of cadmium in the human body can result in chronic toxicity. Melatonin as a free radical scavenger has anti-inflammatory, and even anti-cancer and antiapoptotic functions. Aim: In this work, we evaluated the protective effect of melatonin on human sperm parameters treated by cadmium. Study Setting and Design: This was an experimental study carried out from May to December 2019. Materials and Methods: A total of 41 fresh semen samples were collected from fertile men and were divided into 4 groups: (1) control, (2) sperm +25 Nm cd, (3) sperm +25 nM cd +0.1 mM melatonin,(4) sperm +0.1 mM melatonin treated for 60 min. In all groups, semen analysis was performed for motility, viability and DNA fragmentation index (DFI). Statistical Analysis: The groups were compared using the ANOVA test. Results: The group treated with cadmium showed a significant decrease in rapid and slow motility, and survival rate compared with the control group (P < 0.05). However, the degree of DFI and sperm with non-progressive motility in the group treated with cadmium had a significant increase compared to the control (P < 0.05). The use of melatonin significantly improved sperm parameters such as motility, survival rate and decreased sperm DFI with non-progressive motility. Conclusions: The use of melatonin reduces the amount of cadmium damage in human sperm in vitro.
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