Journal of Human Reproductive Sciences

ORIGINAL ARTICLE
Year
: 2016  |  Volume : 9  |  Issue : 1  |  Page : 35--40

Association of large scale 4977-bp DQcommonDQ deletions in sperm mitochondrial DNA with asthenozoospermia and oligoasthenoteratozoospermia


Prafulla S Ambulkar1, Ajay R Chuadhari2, Asoke K Pal1 
1 Department of Anatomy, Human Genetic Division, Mahatma Gandhi Institute of Medical Sciences, Wardha, Maharashtra, India
2 Department of Physiology, Mahatma Gandhi Institute of Medical Sciences, Wardha, Maharashtra, India

Correspondence Address:
Dr. Asoke K Pal
Department of Anatomy, Human Genetic Division, Mahatma Gandhi Institute of Medical Sciences, Sevagram, Wardha - 442102, Maharashtra
India

OBJECTIVE: To determine the association of large-scale mitochondrial DNA (mtDNA) deletions with abnormal sperm or abnormal flagellar movement of human spermatozoa in asthenozoospermia and oligoasthenoteratozoospermia (OAT) subjects using percoll gradients fractionation and long-range polymerase chain reaction (PCR). DESIGN: We investigated sixty infertile men and thirty normal healthy fertile controls. Of sixty infertile men, 39 were asthenozoospermia and 21 were OAT. MATERIALS AND METHODS: Percoll gradients discontinuous technique was used for separation of spermatozoa on the basis of their motility. Long-range PCR was used for detection of “common ” 4977-bp deletions, and primer shift technique was used for confirmation of deletions. RESULTS: Overall fourteen subjects (14/60; 23.3%) of which eight (8/39; 20.5%) asthenozoospermia and six (6/21; 28.6%) OAT had shown deletions of 4977-bp. Deletions were more common (23.3%) in 40% fraction than 60% (11.6%) and 80% (5%) fractions. Sequencing results had shown deleted region of mtDNA. CONCLUSION: Abnormal spermatozoa had more number of mtDNA deletions than normal sperm, and abnormal spermatozoa had lost genes for the oxidative phosphorylation. Our findings suggest that large-scale 4977-bp mtDNA deletions in the spermatozoa from the infertile subjects cause the asthenozoospermic and OAT pathophysiological conditions in infertile males.


How to cite this article:
Ambulkar PS, Chuadhari AR, Pal AK. Association of large scale 4977-bp "common" deletions in sperm mitochondrial DNA with asthenozoospermia and oligoasthenoteratozoospermia.J Hum Reprod Sci 2016;9:35-40


How to cite this URL:
Ambulkar PS, Chuadhari AR, Pal AK. Association of large scale 4977-bp "common" deletions in sperm mitochondrial DNA with asthenozoospermia and oligoasthenoteratozoospermia. J Hum Reprod Sci [serial online] 2016 [cited 2022 Jun 28 ];9:35-40
Available from: https://www.jhrsonline.org/article.asp?issn=0974-1208;year=2016;volume=9;issue=1;spage=35;epage=40;aulast=Ambulkar;type=0