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 Indian J Med Microbiol  
 

Figure 3: Androgen dependency analysis of CD52 using quantitative real-time polymerase chain reaction. Mice were castrated, and the cauda epididymides were isolated for RNA extraction after 6 h, 1 day and 3 day after gonadectomy (6 h G, 1 d G and 3 d G) and also 1 day and 3 day after castration with testosterone replacement therapy (1d+ T, 3d+ T). The results showed that CD52 was slightly upregulated at 6 h and significantly downregulated 1–3 days after castration (P < 0.05). The expression levels after testosterone injection were higher than mice day 1 and 3 castration without testosterone. Each level of expression was normalised against beta-actin. Error bars represent standard error of the mean (n = 3)

Figure 3: Androgen dependency analysis of CD52 using quantitative real-time polymerase chain reaction. Mice were castrated, and the cauda epididymides were isolated for RNA extraction after 6 h, 1 day and 3 day after gonadectomy (6 h G, 1 d G and 3 d G) and also 1 day and 3 day after castration with testosterone replacement therapy (1d+ T, 3d+ T). The results showed that CD52 was slightly upregulated at 6 h and significantly downregulated 1–3 days after castration (<i>P</i> < 0.05). The expression levels after testosterone injection were higher than mice day 1 and 3 castration without testosterone. Each level of expression was normalised against beta-actin. Error bars represent standard error of the mean (<i>n</i> = 3)