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Figure 4: Efferent duct ligation (EDL) analysis using quantitative real-time polymerase chain reaction to examine the dependency of CD52 towards lumicrine factors in the epididymis. Mice efferent ducts were ligated for 6 h, 1 day, 3 day and 5 day after EDL and control (unligated). The result showed that CD52 was slightly upregulated at 6 h and significantly downregulated at day 1 to 5 (P < 0.05). Day 5 was the lowest level expression. Each level of expression was normalised to beta-actin. The error bars represent the standard error of the mean (n = 3) |
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